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SRT and Zenix SEC Phases - A Total Solution for Size Exclusion Separation


Zenix SEC
Highest separation efficiency and resolution
Made of 3 µm silica particles, Zenix SEC packing is a breakthrough technology for size exclusion chromatography. Combination of 3 µm silica particle size and proprietary surface technology enables Zenix SEC phases achieved highest separation efficiency and resolution for biological molecules and water soluble polymers. Available pore sizes of Zenix packings are 100, 150 and 300Å.

 




SRT SEC
High Capacity and High Resolution Size Exclusion Phases
Utilizing proprietary surface technologies, SRT SEC phases are made of the uniform, hydrophilic, and neutral nanometer thick films chemically bonded on the high purity and enhanced mechanical stability silica. This proprietary surface technology results in excellent column-to-column reproducibility. The nature of the chemical bonding and the maximum bonding density of the thin film benefit SRT SEC phases with high stability and negligible non-specific interactions. Along with the widest pore size selection of 100, 150, 300, 500, 1000 and 2000Å, SRT SEC packings have highest capacity that enables highest resolution.

Sepax Proteomix Ion-Exchange Columns






Non-porous Proteomix Ion-exchange Phases
High Resolution and High Capacity
Proteomix ion-exchange phases have been innovatively developed for achieving high ion-exchange capacity, a breakthrough technology for non-porous resins. Proteomix packing support is composed of a rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) non-porous bead with the particle size selections of 1, 1.7, 3, 5 and 10 µm. The PS/DVB resin surface is grafted with a highly hydrophilic, neutral polymer thin layer with the thickness in the range of nanometer. The hydrophobic PS/DVB resin surface is totally covered by such a hydrophilic coating that eliminates non-specific bindings with biological analytes, leading to high efficiency and high recovery separations for biological molecules. On the top of the hydrophilic layer, ion-exchange functional groups are attached via a proprietary chemistry, resulting in high capacity ion-exchange layer.
The uniqueness of non-porous Proteomix ion-exchange phases is that they combine increased capacity and resolution with their intrinsic advantages of high efficiency and high separation speed. Proteomics ion-exchange phases are especially suitable for high resolution, high efficiency and high recovery separations of proteins, oligonucleotides, peptides, polysaccharides, cell lysates, nanoparticles and nanotubes.


Porous Proteomix Ion-exchange Phases
Porous Proteomix packing support is composed of a rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) particle with a pore size of 500 Å. The surface is grafted with a highly hydrophilic, neutral polymer thin layer, and further chemically bonded with a layer of ion-exchange functional groups. Four ion-exchange phases (SCX, WCX, SAX and WAX) are specially designed for high capacity and high recovery separations of proteins, oligonucleotides, carbohydrates, and peptides. The porous Proteomix resins have particle size of 5 and 10 µm for analytical separation, and 30 and 50 µm for preparative separation.

Antibody Separation











Sepax Antibodix Column
Antibodix columns are specially designed for high resolution, high efficiency and high recovery separations of antibodies. The packing support is composed of a rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) non-porous bead. The non-porous resin has particle size of 1.7, 3, 5 and 10 µm. The PS/DVB resin surface is grafted with a highly hydrophilic, neutral polymer thin layer with the thickness in the range of nanometer. On the top of the hydrophilic layer, weak cation-exchange functional groups are attached via a proprietary chemistry, resulting in high capacity ion-exchange layer.

Carbohydrate Separation

 

 

 

 


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Sepax Carbomix NP Columns

Carbomix phases are composed of spherical, low cross-linking polystyrene/ divinylbenzene (PS/DVB) particles with their surfaces modified with sulfonic acid (-SO3H), followed by chelating of calcium ions (Ca+2) or other metal ions. The PS/DVB support has cross-linking degree of 5%, 8% and 10%. The standard Carbomix packings for analytical applications are 5 µm uniform particles. Carbomix resins of 10 µm and above particle size are manufactured for preparative separation applications. Carbomix resins have been specifically designed for high resolution separation of carbohydrates, organic acids, peptides, and nucleic acids.

Capillary Electrophoresis

 











Capillary Electrophoresis
Nanofilm capillary coatings are Sepax patented surface coating technologies that have been developed to coat the capillary tubes with uniform polymer thin films. The polymer film thickness can be well controlled in the range from a few nanometers to a few hundreds of nanometers. The standard Nanofilm coated capillaries have inner diameter (ID) selections of 75, 50 and 25 µm. Our innovative coating process can be readily applied to capillary tubes with even thinner inner diameter down to 10 µm for special applications. Nanofilm coatings include polyacrylamide (PAAm), polyethylene glycol (PEG), PAAm-PEG copolymer, ionic polymers and customer-specific coatings. Elimination of non-specific interactions with biological molecules enables Nanofilm coated capillaries ideal for DNA separation and sequencing, protein separation, protein-protein interaction studies.

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